• Format/Info:
• Positions must correspond to the selected reference genome.
• For SNVs, the position refers to the exact location of the variant.
• For InDels, the two positions refer to the start and end position of the sequence region that is substituted with the given variant sequence.
• Inserted bases can be specified either as a base sequence (e.g., AGT) or as the number of inserted bases (e.g., 3)
• Allowed format: ChrY:2655470
• Optional: upload a VCF (same chromosome as your variant) to spike additional variants into the reference sequence before primer design (patient-specific background).

• Format/Info:
• Positions must correspond to the selected reference genome and sequence type.
• Optional amplicon check (Dicey: None, genome, or transcriptome) is configured in the top settings row.
• Optional Known SNPs (gnomAD) check queries Ensembl for common variants (MAF > 1%) overlapping the displayed design window and flags primer pairs where common SNPs overlap binding sites.
• For SNVs, the position refers to the exact location of the variant.
• For InDels, the two positions refer to the start and end position of the sequence region that is substituted with the given variant sequence.
• Inserted bases can be specified either as a base sequence (e.g., AGT) or as the number of inserted bases (e.g., 3)

Accepted formats:
CGACT[A>G]GC
CGACT[AC/-]GC
CGACT[-/TT]GC
CGACT[ACA/TT]GC

A substituted by G (SNV)
AC deleted
TT inserted
ACA substituted by TT (DelIns)